Archer1
Archaerhodopsin variant functioning as both a voltage indicator and a light-activated neuronal silencer.
Category: Opsin GEVI · Published: 2014 · Nature Communications 2014
Speed: τ_on=0.5 ms, τ_off=0.5 ms
Dynamic Range: 85% ΔF/F per 100mV
Sensitivity: 33% ΔF/F per AP
Brightness: 10× vs arch
Research Papers (6)
- Archaerhodopsin variants with enhanced voltage-sensitive fluorescence in mammalian and Caenorhabditis elegans neurons — Flytzanis NC, Bedbrook CN, Chiu H, Engqvist MKM, Xiao C, Chan KY, Sternberg PW, Arnold FH, Gradinaru V, Nature Communications 2014
- A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters — Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, et al., Nature Chemical Biology 2018
- Comparative Evaluation of Genetically Encoded Voltage Indicators — Bando Y, Sakamoto M, Kim S, Ayzenshtat I, Yuste R, Cell Reports 2019
- Screening and Cellular Characterization of Genetically Encoded Voltage Indicators Based on Near-Infrared Fluorescent Proteins — Monakhov MV, Matlashov ME, Colavita M, Song C, Shcherbakova DM, Antic SD, Verkhusha VV, Knöpfel T, ACS Chemical Neuroscience 2020
- Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants — Nikolaev DM, et al., ACS Physical Chemistry Au 2024
- Semirational Protein Engineering Yields Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators with Enhanced Brightness and Red Shifted Absorption Bands — Nikolaev DM, et al., Chem & Bio Engineering 2025