ASAP2s
Higher-sensitivity ASAP1 variant that trades speed for larger fluorescence responses.
Category: VSD-cpGFP GEVI · Published: 2017 · eLife 2017
Speed: τ_on=5.2 ms, τ_off=24 ms
Dynamic Range: -38.7% ΔF/F per 100mV
Sensitivity: 12.2% ΔF/F per AP
Brightness: 1.11× vs asap3
Research Papers (12)
- Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators — Chamberland S, Yang HH, et al., eLife 2017
- In Vitro Testing of Voltage Indicators: Archon1, ArcLightD, ASAP1, ASAP2s, ASAP3b, Bongwoori-Pos6, BeRST1, FlicR1, and Chi-VSFP-Butterfly — Milosevic MM, et al., eNeuro 2020
- Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice — Villette V, et al., Cell 2019
- Imaging of Evoked Cortical Depolarizations Using Either ASAP2s, or chi-VSFP, or Di-4-Anepps, or Autofluorescence Optical Signals — Milicevic KD, et al., Journal of Integrative Neuroscience 2023
- CaMKII Measures the Passage of Time to Coordinate Behavior and Motivational State — Thornquist SC, et al., Neuron 2019
- Biochemical evidence accumulates across neurons to drive a network-level eruption — Thornquist SC, et al., Molecular Cell 2021
- Drosophila TRPγ is required in neuroendocrine cells for post-ingestive food selection — Dhakal S, et al., eLife 2022
- Triclosan disrupts immune cell function by depressing Ca2+ influx following acidification of the cytoplasm — Sangroula S, et al., Toxicology and Applied Pharmacology 2020
- Real-time detection of changes in yeast plasma membrane potential using genetically encoded voltage indicator proteins — Limapichat W, et al., FEMS Yeast Research 2020
- A dark quencher genetically encodable voltage indicator (dqGEVI) exhibits high fidelity and speed — Alich TC, et al., Proceedings of the National Academy of Sciences 2021
- Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy — Liu Z, Lu X, et al., Cell 2022
- Physiological features of parvalbumin-expressing GABAergic interneurons contributing to high-frequency oscillations in the cerebral cortex — Milicevic KD, et al., Current Research in Neurobiology 2023