VSFP2.3
The first VSFP variant to achieve reliable in vivo cortical voltage imaging in transgenic mice.
Category: VSD-FRET GEVI · Published: 2008 · PLoS ONE 2008
Speed: τ_on=46.7 ms, τ_off=49.3 ms
Dynamic Range: -8.3% ΔF/F per 100mV
Sensitivity: 0.2% ΔF/F per AP
Brightness: 1.74× vs EGFP
Research Papers (8)
- Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements — Lundby A, Mutoh H, Dimitrov D, Akemann W, Knöpfel T, PLoS ONE 2008
- Biophysical characterization of the fluorescent protein voltage probe VSFP2.3 based on the voltage-sensing domain of Ci-VSP — Lundby A, Akemann W, Knöpfel T, European Biophysics Journal 2010
- Spectrally-resolved response properties of the three most advanced FRET based fluorescent protein voltage probes — Mutoh H, Perron A, Dimitrov D, Iwamoto Y, Akemann W, Chudakov DM, Knöpfel T, PLoS ONE 2009
- Imaging brain electric signals with genetically targeted voltage-sensitive fluorescent proteins — Akemann W, Mutoh H, Perron A, Rossier J, Knöpfel T, Nature Methods 2010
- Exploration of genetically encoded voltage indicators based on a chimeric voltage sensing domain — Mishina Y, Mutoh H, Song C, Knöpfel T, Frontiers in Molecular Neuroscience 2014
- Comparative performance of a genetically-encoded voltage indicator and a blue voltage sensitive dye for large scale cortical voltage imaging — Mutoh H, Mishina Y, Gallero-Salas Y, Knöpfel T, Frontiers in Cellular Neuroscience 2015
- Sensing Cardiac Electrical Activity With a Cardiac Myocyte-Targeted Optogenetic Voltage Indicator — Chang Liao ML, et al., Circulation Research 2015
- Electrotonic coupling of excitable and nonexcitable cells in the heart revealed by optogenetics — Quinn TA, et al., Proceedings of the National Academy of Sciences 2016