VSFP3.1
Third-generation Ci-VSP voltage sensor using a single Cerulean fluorescent protein, derived from VSFP2 by removing the FRET acceptor, achieving the fastest response kinetics reported among fluorescent protein-based voltage indicators at the time.
Category: VSD-FRET GEVI · Published: 2009 · Front. Mol. Neurosci. 2009
Speed: τ_on=1.3 ms, τ_off=undefined ms
Dynamic Range: -1.9% ΔF/F per 100mV
Brightness: 0.79× vs EGFP
Research Papers (5)
- Second and third generation voltage-sensitive fluorescent proteins for monitoring membrane potential — Perron A, Mutoh H, Akemann W, Ghimire Gautam S, Dimitrov D, Iwamoto Y, Knöpfel T, Frontiers in Molecular Neuroscience 2009
- Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements — Lundby A, Mutoh H, Dimitrov D, Akemann W, Knöpfel T, PLoS ONE 2008
- Red-shifted voltage sensitive fluorescent proteins — Perron A, Mutoh H, Launey T, Knöpfel T, Chemistry & Biology 2009
- Effect of voltage sensitive fluorescent proteins on neuronal excitability — Akemann W, Lundby A, Mutoh H, Knöpfel T, Biophysical Journal 2009
- Membrane 'potential-omics': toward voltage imaging at the cell population level in roots of living plants — Matzke AJ, Matzke M, Frontiers in Plant Science 2013