GEVIBench › All sensors › Ace2N-mNeon
Ace2N-mNeon
Opsin-fluorescent protein FRET voltage indicator pairing an Ace rhodopsin with mNeonGreen, optimized for fast sub-millisecond responses and high-fidelity spike detection.
- Category: eFRET GEVI
- Published: 2015-12-11 — Science 2015
- Response polarity: negative-going (fluorescence decreases on depolarization)
- Spectrum: excitation peak 506 nm, emission peak 517 nm (Ace2N-mNeon)
- Two-photon compatible: no
- Tags: Ace opsin
Headline values (as shown in the GEVIBench table)
| Metric | Value | Definition |
|---|---|---|
| Brightness (B/B_EGFP) | 2.73× EGFP | Molecular brightness relative to EGFP (extinction coefficient × quantum yield, or a measured ratio). Sensors not measured against EGFP directly are placed through a chain of pairwise brightness comparisons, combined by geometric mean. |
| Kinetics τ_on / τ_off (ms) | 2.2 / 3.8 ms | Activation / decay time constants in ms (amplitude-weighted mean τ for multi-exponential fits). The value shown averages the measurements made at physiological temperature (≥33 °C); if there are none, it is the measurement closest to 33 °C. |
| ΔF/F per 100 mV | -17.9% (median of 6 measurements: −11.6%) | Steady-state fluorescence change for a 100 mV depolarization step (−70 → +30 mV); the sign gives the polarity. The table shows the first listed measurement; the median across measurements (one-photon preferred) is used for sorting. |
| ΔF/F per mV | 0.091% | Subthreshold sensitivity: fluorescence change per mV near rest (−90 to −50 mV). Where no value is reported directly, it is estimated from the F–V curve. |
| ΔF/F per AP | 5% (median of 4 measurements: 4.95%) | Fluorescence change per single action potential in neurons. The table shows the first listed measurement; the median across measurements (one-photon preferred) is used for sorting. |
| AP width (FWHM, ms) | 3.9 ms | Full width at half maximum of the optical spike waveform. |
| Photostability t75% @ 100 mW/mm² | 11 s | Time for fluorescence to fall to 75% of its initial value under one-photon illumination, linearly dose-scaled to 100 mW/mm² (t50% is used where the 75% crossing falls inside a fast initial transient). Two-photon curves are not dose-scaled and are excluded. |
| Papers using it (N_used) | 6 | Number of published research papers, tracked by GEVIBench, that used the sensor for an experiment (a popularity / track-record measure). |
Measurements and sources
Kinetics
- τ_on 1.36 ms, τ_off 2.14 ms at 22°C — Gong 2015, Table S2
- τ_on 2.2 ms, τ_off 3.8 ms at 33-34°C — Villette 2019, Table 1
- τ_on 2.33 ms, τ_off 2.54 ms at 22°C — Kannan 2018, Table S1
ΔF/F per 100 mV
- -17.9% (peak), 1P — Gong 2015, Fig. 1D
- -5%, 1P — Gong 2015, Fig. 1D
- -19.4% (peak), 1P — Gong 2015, Fig. S6C
- -9%, 1P — Villette 2019, Table 1
- -11.6%, 1P — Kannan 2018, Fig. 2b
- -11.5%, 1P — Hasseman 2022, Fig. 1
ΔF/F per mV (subthreshold)
- 0.091%/mV — Abdelfattah 2023, Fig. S5D
ΔF/F per action potential
- 5%, 1P — Villette 2019, Table 1
- 5%, 1P — Hasseman 2022, Fig. 2
- 4.9%, 1P — Liu 2021, Table S3
- 4.3%, 1P — Kannan 2018, Fig. 3d
AP width (FWHM)
- 3.9 ms at 500 Hz sampling, Drosophila, PPL1-α'2α2 dopaminergic neuron, in vivo, 1P, 25°C — Kannan 2018, Fig. S15
Brightness comparisons
- 2.76× the brightness of EGFP — Shaner 2013, Table 1
- 0.9090909090909091× the brightness of Ace2N-mNeon2 — Kannan 2022, Fig. S11A
- 5.988023952095808× the brightness of ASAP3 — Villette 2019, Table 1
- 5.555555555555555× the brightness of FlicR1 — Kannan 2018, Fig. 1j
- 5.555555555555555× the brightness of FlicR2 — Kannan 2018, Fig. 1j
- 1.4285714285714286× the brightness of pAce — Kannan 2022, Fig. S11A
- 1.4084507042253522× the brightness of VARNAM — Kannan 2018, Fig. 1j
- 0.25× the brightness of Voltron — Abdelfattah 2019, Fig. 1F
Photobleaching
- t75% = 92 s, 1P — Kannan 2022, Fig. S11B
- t75% = 49 s at 31 mW/mm², 1P — Liu 2021, Ext. Fig. 4g
- t75% = 32 s at ~23 mW/mm² (475/50 nm), 1P — Abdelfattah 2019, Fig. 1H
- t75% = 31 s at 15 mW/mm², 1P — Kannan 2018, Fig. S7a
- t75% = 104 s at 13 mW/mm², 1P — Piatkevich 2018, Fig. 2i
Fluorescence–voltage (F–V) curve
ΔF/F (%) at each membrane potential, normalized to 0 at −70 mV: -100 mV: 5.4%; -70 mV: 0%; -50 mV: -3.6%; -30 mV: -7.2%; 0 mV: -12.6%; 30 mV: -17.9% — Gong 2015, Fig. 1D
Research papers using Ace2N-mNeon (6)
- High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor — Gong Y, et al., Science 2015 (Rat hippocampal neurons, in vitro; mouse V1 cortex, in vivo; Drosophila olfactory neurons, in vivo)
- Long-term optical brain imaging in live adult fruit flies — Huang C, et al., Nature Communications 2018 (Drosophila, dopamine neurons, in vivo)
- Comparative Evaluation of Genetically Encoded Voltage Indicators — Bando Y, et al., Cell Reports 2019 (Mouse hippocampal neurons, in vitro; mouse visual cortex, in vivo)
- Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators — Chamberland S, et al., eLife 2017 (Drosophila visual neurons (L2), in vivo; rat hippocampal organotypic slices, in vitro)
- Exploring Bioelectricity with Ace2N-mNeon during Zebrafish Embryogenesis — Wu Z, et al., bioRxiv 2024 (Zebrafish embryos, neurons and cardiomyocytes, in vivo)
- Imaging high-frequency voltage dynamics in multiple neuron classes of behaving mammals — Haziza S, et al., Cell 2025 (Mouse, cortex, in vivo)
Data and citation
- llms.txt — summary for language models
- llms-full.txt — every sensor in full (Markdown)
- gevis.json — all values and measurements with sources (JSON)
- gevis.csv — one row per sensor (CSV)
Cite GEVIBench as: Adam E. Cohen and Daozheng Gong (2026). Voltage imaging: A practical guide. Neuron. https://doi.org/10.1016/j.neuron.2026.08.002
Generated from the GEVIBench database on 2026-10-12.