ArcLight
Classic GEVI using a VSD fused to pH-sensitive GFP.
Category: VSD-single FP · Published: 2012 · Neuron 2012
Speed: τ_on=9 ms, τ_off=17 ms
Dynamic Range: -35% ΔF/F per 100mV
Sensitivity: 3.2% ΔF/F per AP
Brightness: 1.097× vs EGFP
Research Papers (53)
- Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe — Jin L, Han Z, Platisa J, Wooltorton JRA, Cohen LB, Pieribone VA, Neuron 2012
- Genetically targeted optical electrophysiology in intact neural circuits — Cao G, et al., Cell 2013
- Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics — Han Z, et al., PLoS One 2013
- Rapid cellular phenotyping of human pluripotent stem cell-derived cardiomyocytes using a genetically encoded fluorescent voltage sensor — Leyton-Mange JS, et al., Stem Cell Reports 2014
- Imaging neural spiking in brain tissue using FRET-opsin protein voltage sensors — Gong Y, et al., Nature Communications 2014
- Mechanistic studies of the genetically encoded fluorescent protein voltage probe ArcLight — Han Z, et al., PLoS One 2014
- Monitoring brain activity with protein voltage and calcium sensors — Storace DA, et al., Scientific Reports 2015
- Two-photon lifetime imaging of voltage indicating proteins as a probe of absolute membrane voltage — Brinks D, et al., Biophysical Journal 2015
- Monitoring human-induced pluripotent stem cell-derived cardiomyocytes with genetically encoded calcium and voltage fluorescent reporters — Shinnawi R, et al., Stem Cell Reports 2015
- Dual optical recordings for action potentials and calcium handling in induced pluripotent stem cell models of cardiac arrhythmias — Song L, et al., Stem Cells Translational Medicine 2015
- Expression and testing in plants of ArcLight, a genetically-encoded voltage indicator used in neuroscience research — Matzke AJ, et al., BMC Plant Biology 2015
- Single-molecule fluorimetry and gating currents inspire an improved optical voltage indicator (ArcLightning) — Treger JS, et al., eLife 2015
- Ziram, a pesticide associated with increased risk for Parkinson's disease, differentially affects the presynaptic function of aminergic and glutamatergic nerve terminals — Martin CA, et al., Experimental Neurology 2016
- Optogenetic monitoring of synaptic activity with genetically encoded voltage indicators — Nakajima R, et al., Frontiers in Synaptic Neuroscience 2016
- Directed evolution of key residues in fluorescent protein inverses the polarity of voltage sensitivity in ArcLight (Marina) — Platisa J, et al., ACS Chemical Neuroscience 2017
- Genetically expressed voltage sensor ArcLight for imaging large scale cortical activity in the anesthetized and awake mouse — Borden PY, et al., Neurophotonics 2017
- Attenuation of synaptic potentials in dendritic spines — Kwon T, et al., Cell Reports 2017
- Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition (Bongwoori-R3) — Lee S, et al., Scientific Reports 2017
- MicroRNA-92a is a circadian modulator of neuronal excitability in Drosophila — Chen X, et al., Nature Communications 2017
- Human induced pluripotent stem cell-derived cardiac cell sheets expressing genetically encoded voltage indicator for pharmacological and arrhythmia studies — Shaheen N, et al., Stem Cell Reports 2018
- A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice (Ilmol) — Yi B, et al., Scientific Reports 2018
- Mapping of excitatory and inhibitory postsynaptic potentials of neuronal populations in hippocampal slices using the GEVI, ArcLight — Nakajima R, et al., Journal of Physics D: Applied Physics 2018
- Optical consequences of a genetically-encoded voltage indicator with a pH sensitive fluorescent protein — Kang BE, et al., Neuroscience Research 2019
- Comparative evaluation of genetically encoded voltage indicators — Bando Y, et al., Cell Reports 2019
- Using genetically encoded voltage indicators (GEVIs) to study the input-output transformation of the mammalian olfactory bulb — Storace DA, et al., Frontiers in Cellular Neuroscience 2019
- Single-cell RNA-sequencing and optical electrophysiology of human iPSC-derived cardiomyocytes reveal discordance between cardiac subtype-associated gene expression and electrophysiological phenotypes — Biendarra-Tiegs SM, et al., Stem Cells and Development 2019
- Characterization of the CACNA1C-R518C missense mutation in the pathobiology of long-QT syndrome using human iPSC cardiomyocytes — Estes SI, et al., Circulation: Genomic and Precision Medicine 2019
- Human induced pluripotent stem cell line with genetically encoded fluorescent voltage indicator generated via CRISPR — Sun YH, et al., Stem Cells 2020
- Human iPSC-derived non-cardiomyocytes modulate cardiac electrophysiological maturation through connexin 43-mediated cell-cell interactions — Biendarra-Tiegs SM, et al., Stem Cells and Development 2020
- Real-time detection of changes in yeast plasma membrane potential using genetically encoded voltage indicator proteins — Limapichat W, et al., FEMS Yeast Research 2020
- In vitro testing of voltage indicators: Archon1, ArcLightD, ASAP1, ASAP2s, ASAP3b, Bongwoori-Pos6, BeRST1, FlicR1, and Chi-VSFP-Butterfly — Milosevic MM, et al., eNeuro 2020
- Triclosan disrupts immune cell function by depressing Ca2+ influx following acidification of the cytoplasm — Sangroula S, et al., Toxicology and Applied Pharmacology 2020
- Analysing bioelectrical phenomena in the Drosophila ovary with genetic tools — Schotthoefer SK, et al., BMC Developmental Biology 2020
- Promise and potential peril with lumacaftor for the trafficking defective type 2 long-QT syndrome-causative variants — O'Hare BJ, et al., Circulation: Genomic and Precision Medicine 2020
- GEVI cell-type specific labelling and a manifold learning approach provide evidence for lateral inhibition at the population level in mouse hippocampal CA1 — Nakajima R, et al., European Journal of Neuroscience 2021
- Improving the flexibility of genetically encoded voltage indicators via intermolecular FRET — Leong LM, et al., Biophysical Journal 2021
- Mechanism of ArcLight derived GEVIs involves electrostatic interactions that can affect proton wires — Kang BE, et al., Biophysical Journal 2021
- Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation — Rieger M, et al., Nature Communications 2021
- Studying synaptically evoked cortical responses ex vivo with combination of single neuron recording and population voltage imaging — Jang J, et al., Frontiers in Neuroscience 2021
- Visualizing oscillations in brain slices with genetically encoded voltage indicators — Rhee JK, et al., Frontiers in Neuroanatomy 2021
- Simultaneous two-photon imaging of action potentials and subthreshold inputs in vivo — Bando Y, et al., Nature Communications 2021
- Voltage imaging in the olfactory bulb using transgenic mouse lines expressing ArcLight — Platisa J, et al., Scientific Reports 2022
- Conserved amino acids residing outside the voltage field can shift the voltage sensitivity and increase the signal speed and size of Ciona based GEVIs — Sepehri Rad M, et al., Frontiers in Cell and Developmental Biology 2022
- Recombinant adeno-associated viral vector-mediated gene transfer of hTBX18 generates pacemaker cells from ventricular cardiomyocytes — Farraha M, et al., International Journal of Molecular Sciences 2022
- Generation of human iPSC line encoding for ArcLight A242 — Vivekanandan R, et al., Stem Cell Research 2023
- Voltage to calcium transformation enhances direction selectivity in Drosophila T4 neurons — Mishra A, et al., Journal of Neuroscience 2023
- Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators — Chamberland S, et al., eLife 2017
- Imaging different cell populations in the mouse olfactory bulb using ArcLight — Leong LM, et al., Neurophotonics 2024
- Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 — Petruskeviciu-te A, et al., Stem Cell Research 2025
- Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice — Villette V, et al., Cell 2019
- All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins — Hochbaum DR, et al., Nature Methods 2014
- High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor — St-Pierre F, et al., Nature Neuroscience 2014
- A bright and fast red fluorescent protein voltage indicator that reports neuronal activity in organotypic brain slices — Abdelfattah AS, et al., Journal of Neuroscience 2016