ASAP1
First ASAP sensor, establishing the VSD/cpGFP architecture for fast, negative-going fluorescence responses to voltage.
Category: VSD-cpGFP GEVI · Published: 2014 · Nature Neuroscience 2014
Speed: τ_on=2.1 ms, τ_off=2 ms
Dynamic Range: -17.5% ΔF/F per 100mV
Sensitivity: 6.3% ΔF/F per AP
Brightness: 1× vs asap2s
Research Papers (17)
- High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor — St-Pierre F, Marshall JD, Yang Y, Gong Y, Schnitzer MJ, Lin MZ, Nature Neuroscience 2014
- A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices — Abdelfattah AS, Farhi SL, Bharioke A, Bharioke A, Boyden ES, Campbell RE, Journal of Neuroscience 2016
- Biophysical Characterization of Genetically Encoded Voltage Sensor ASAP1: Dynamic Range Improvement — Lee EEL, Bezanilla F, Biophysical Journal 2017
- Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators — Chamberland S, et al., eLife 2017
- In Vitro Testing of Voltage Indicators: Archon1, ArcLightD, ASAP1, ASAP2s, ASAP3b, Bongwoori-Pos6, BeRST1, FlicR1, and Chi-VSFP-Butterfly — Milosevic MM, et al., eNeuro 2020
- Comparative Evaluation of Genetically Encoded Voltage Indicators — Bando Y, et al., Cell Reports 2019
- Optical interrogation of neuronal circuitry in zebrafish using genetically encoded voltage indicators — Miyazawa H, et al., Scientific Reports 2018
- Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors — Silic MR, Zhang G, Journal of Visualized Experiments 2018
- Real-time detection of yeast membrane potential using ASAP1 voltage indicator — Limapichat W, et al., FEMS Yeast Research 2020
- In vivo wide-field voltage imaging in zebrafish with voltage-sensitive dye and genetically encoded voltage indicator — Hiyoshi K, et al., Development, Growth & Differentiation 2021
- Enabling comprehensive optogenetic studies of mouse hearts by simultaneous opto-electrical panoramic mapping and stimulation — Rieger M, et al., Nature Communications 2021
- In Vitro Matured Human Pluripotent Stem Cell-Derived Cardiomyocytes Form Grafts With Enhanced Structure and Function in Injured Hearts — Dhahri W, et al., Circulation 2022
- Zebrafish Embryos Display Characteristic Bioelectric Signals during Early Development — Silic MR, et al., Cells 2022
- Optogenetic Reporters Delivered as mRNA Facilitate Repeatable Action Potential and Calcium Handling Assessment in Human iPSC-Derived Cardiomyocytes — Yiangou L, et al., Stem Cells 2022
- A fully-automated low-cost cardiac monolayer optical mapping robot — Lee P, et al., Frontiers in Cardiovascular Medicine 2023
- Protocol for dual-optical mapping of voltage and calcium sensors in human pluripotent stem cell-derived cardiomyocytes — Alibhai FJ, et al., STAR Protocols 2025
- Absolute Membrane Potential Recording with ASAP-Type Genetically Encoded Voltage Indicators Using Fluorescence Lifetime Imaging — Nair AG, et al., ACS Chemical Neuroscience 2025