CAESR

Pioneering opsin-FRET voltage indicator fusing Citrine to the QuasAr2 archaerhodopsin quencher; the prototype of the QuasAr-based eFRET sensor lineage.

Category: eFRET GEVI · Published: 2014 · Nature Communications 2014

Speed: τ_on=4.3 ms, τ_off=4.3 ms

Dynamic Range: -13.1% ΔF/F per 100mV

Sensitivity: 9.7% ΔF/F per AP

Brightness: 2.39× vs EGFP

Research Papers (2)

Bright and fast multicoloured voltage reporters via electrochromic FRET — Zou P, Zhao Y, Douglass AD, Hochbaum DR, Brinks D, Werley CA, Harrison DJ, Campbell RE, Cohen AE, Nature Communications 2014
Two-photon lifetime imaging of voltage indicating proteins as a probe of absolute membrane voltage — Brinks D, Klein AJ, Cohen AE, Biophysical Journal 2015