GEVIBench › All sensors › ElectraOFF
ElectraOFF
Photostable negative-going eFRET GEVI combining Ace2 opsin with mBaoJin(3M), a StayGold-derived bright and photostable green FP.
- Category: eFRET GEVI
- Published: 2025-06-03 — bioRxiv 2025
- Derived from: Ace2N-mNeon2
- Response polarity: negative-going (fluorescence decreases on depolarization)
- Spectrum: excitation peak 500 nm, emission peak 508 nm (ElectraOFF)
- Tags: Ace opsin, mBaoJin, Green
Headline values (as shown in the GEVIBench table)
| Metric | Value | Definition |
|---|---|---|
| Brightness (B/B_EGFP) | 4.86× EGFP | Molecular brightness relative to EGFP (extinction coefficient × quantum yield, or a measured ratio). Sensors not measured against EGFP directly are placed through a chain of pairwise brightness comparisons, combined by geometric mean. |
| Kinetics τ_on / τ_off (ms) | 1.8 / 1.1 ms | Activation / decay time constants in ms (amplitude-weighted mean τ for multi-exponential fits). The value shown averages the measurements made at physiological temperature (≥33 °C); if there are none, it is the measurement closest to 33 °C. |
| ΔF/F per 100 mV | -13% (median of 2 measurements: −13%) | Steady-state fluorescence change for a 100 mV depolarization step (−70 → +30 mV); the sign gives the polarity. The table shows the first listed measurement; the median across measurements (one-photon preferred) is used for sorting. |
| ΔF/F per mV | 0.1% (estimated from the F–V curve) | Subthreshold sensitivity: fluorescence change per mV near rest (−90 to −50 mV). Where no value is reported directly, it is estimated from the F–V curve. |
| ΔF/F per AP | 14.5% | Fluorescence change per single action potential in neurons. The table shows the first listed measurement; the median across measurements (one-photon preferred) is used for sorting. |
| AP width (FWHM, ms) | 6.4 ms | Full width at half maximum of the optical spike waveform. |
| Photostability t75% @ 100 mW/mm² | 1.1 min | Time for fluorescence to fall to 75% of its initial value under one-photon illumination, linearly dose-scaled to 100 mW/mm² (t50% is used where the 75% crossing falls inside a fast initial transient). Two-photon curves are not dose-scaled and are excluded. |
| Papers using it (N_used) | 2 | Number of published research papers, tracked by GEVIBench, that used the sensor for an experiment (a popularity / track-record measure). |
Measurements and sources
Kinetics
- τ_on 1.75 ms, τ_off 1.14 ms at 23-25°C — Zhang 2025, Fig. S3d
ΔF/F per 100 mV
- -13%, 1P — Zhang 2025, Fig. S3b
- -18%, 2P — Zhang 2025, Fig. 3c
ΔF/F per action potential
- 14.5%, 1P — Zhang 2025, Fig. 1k
AP width (FWHM)
- 6.4 ms at 1000 Hz sampling, Mouse, primary hippocampal neurons, in vitro, 1P, 22°C — Zhang 2025, Fig. 1j
Brightness comparisons
- 1.6× the brightness of Ace2N-mNeon2 — Zhang 2025, Fig. 1e
Photobleaching
- t75% = 1370 s at 5 mW/mm², 1P — Zhang 2025, Fig. S4h
- curve only (did not reach 75% within the measured window) at 10 mW/mm², 1P — Zhang 2025, Fig. 1h
Fluorescence–voltage (F–V) curve
ΔF/F (%) at each membrane potential, normalized to 0 at −70 mV: -100 mV: 2%; -80 mV: 1%; -70 mV: 0%; -60 mV: -1%; -40 mV: -3%; -20 mV: -6%; 0 mV: -9%; 20 mV: -12%; 40 mV: -15%; 60 mV: -19% — Zhang 2025, Fig. S3b
Research papers using ElectraOFF (2)
- Bright and Photostable Voltage Sensors Derived from mBaoJin — Zhang H, Zhou S, Kuzmicheva TP, Subach OM, Grimm C, Eom M, Baror-Sebban S, Adam Y, Yoon YG, Emilliani V, Subach FV, Piatkevich KD, bioRxiv 2025 (HEK293T cells; HeLa cells; Mouse, primary hippocampal neurons, in vitro; Mouse, primary visual cortex (VIP+ interneurons), in vivo; Mouse, hippocampus CA1, in vivo; CHO cells (two-photon))
- Prolonged Single Neuron Voltage Imaging in Behaving Mammals — Wang Y, Ravasio C, Yuxin Z, Xue H, bioRxiv 2025 (Mouse, hippocampus CA1 pyramidal neurons, striatal cholinergic interneurons, and visual cortex PV interneurons, in vivo)
Data and citation
- llms.txt — summary for language models
- llms-full.txt — every sensor in full (Markdown)
- gevis.json — all values and measurements with sources (JSON)
- gevis.csv — one row per sensor (CSV)
Cite GEVIBench as: Adam E. Cohen and Daozheng Gong (2026). Voltage imaging: A practical guide. Neuron. https://doi.org/10.1016/j.neuron.2026.08.002
Generated from the GEVIBench database on 2026-10-12.