Mermaid
FRET-based GEVI using a VSD paired with coral-derived fluorescent proteins for ratiometric voltage.
Category: VSD-FRET GEVI · Published: 2008 · Nature Methods 2008
Speed: τ_on=8 ms, τ_off=15 ms
Dynamic Range: 40% ΔF/F per 100mV
Sensitivity: 1.6% ΔF/F per AP
Brightness: 1.286× vs EGFP
Research Papers (9)
- Improving membrane voltage measurements using FRET with new fluorescent proteins — Tsutsui H, Karasawa S, Okamura Y, Miyawaki A, Nature Methods 2008
- Visualizing voltage dynamics in zebrafish heart — Tsutsui H, et al., The Journal of Physiology 2010
- Optical action potential screening on adult ventricular myocytes as an alternative QT-screen — Tian Q, et al., Cellular Physiology and Biochemistry 2011
- Neural coding in a single sensory neuron controlling opposite seeking behaviours in Caenorhabditis elegans — Kuhara A, et al., Nature Communications 2011
- Rapid evaluation of a protein-based voltage probe using a field-induced membrane potential change — Tsutsui H, et al., Biochimica et Biophysica Acta 2014
- INaP selective inhibition reverts precocious inter- and motorneurons hyperexcitability in the Sod1-G93R zebrafish ALS model — Benedetti L, et al., Scientific Reports 2016
- Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos — Benedetti L, et al., Journal of Visualized Experiments 2017
- Tracking the sarcoplasmic reticulum membrane voltage in muscle with a FRET biosensor — Sanchez C, et al., The Journal of General Physiology 2018
- Use of FRET-Sensor 'Mermaid' to Detect Subtle Changes in Membrane Potential of Primary Mouse PASMCs — Dartsch RC, et al., Cells 2024