VSFP-Butterfly

FRET-based voltage indicator with a novel 'Butterfly' topology sandwiching the voltage-sensing domain between two fluorescent proteins.

Category: VSD-FRET GEVI · Published: 2012 · J. Neurophysiol. 2012

Speed: τ_on=7.7 ms, τ_off=89.9 ms

Dynamic Range: -15% ΔF/F per 100mV

Sensitivity: 0.3% ΔF/F per AP

Brightness: 1.74× vs EGFP

Research Papers (28)

Imaging neural circuit dynamics with a voltage-sensitive fluorescent protein — Akemann W, Mutoh H, Perron A, Park YK, Iwamoto Y, Knöpfel T, Journal of Neurophysiology 2012
Exploration of genetically encoded voltage indicators based on a chimeric voltage sensing domain — Mishina Y, Mutoh H, Song C, Knöpfel T, Frontiers in Molecular Neuroscience 2014
Two-photon voltage imaging using a genetically encoded voltage indicator — Akemann W, et al., Scientific Reports 2013
Voltage imaging of waking mouse cortex reveals emergence of critical neuronal dynamics — Scott G, et al., Journal of Neuroscience 2014
Imaging the awake visual cortex with a genetically encoded voltage indicator — Carandini M, et al., Journal of Neuroscience 2015
Validation of optical voltage reporting by the genetically encoded voltage indicator VSFP-Butterfly from cortical layer 2/3 pyramidal neurons in mouse brain slices — Empson RM, et al., Physiological Reports 2015
Cortical entropy, mutual information and scale-free dynamics in waking mice — Fagerholm ED, et al., Cerebral Cortex 2016
Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance — Madisen L, et al., Neuron 2015
Transgenic strategies for sparse but strong expression of genetically encoded voltage and calcium indicators — Song C, et al., International Journal of Molecular Sciences 2017
Imaging of brain slices with a genetically encoded voltage indicator — Quicke P, et al., Methods in Molecular Biology 2017
Cortical signatures of wakeful somatosensory processing — Song C, et al., Scientific Reports 2018
Effects of arousal on mouse sensory cortex depend on modality — Shimaoka D, et al., Cell Reports 2018
Optogenetic sensors in the zebrafish heart: a novel in vivo electrophysiological tool to study cardiac arrhythmogenesis — van Opbergen CJM, et al., Theranostics 2018
Slow moving neural source in the epileptic hippocampus can mimic progression of human seizures — Chiang CC, et al., Scientific Reports 2018
Single-neuron level one-photon voltage imaging with sparsely targeted genetically encoded voltage indicators — Quicke P, et al., Frontiers in Cellular Neuroscience 2019
In vitro testing of voltage indicators: Archon1, ArcLightD, ASAP1, ASAP2s, ASAP3b, Bongwoori-Pos6, BeRST1, FlicR1, and Chi-VSFP-Butterfly — Milosevic MM, et al., eNeuro 2020
Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators — Quicke P, et al., Neurophotonics 2020
Population imaging discrepancies between a genetically-encoded calcium indicator (GECI) versus a genetically-encoded voltage indicator (GEVI) — Zhu MH, et al., Scientific Reports 2021
Studying synaptically evoked cortical responses ex vivo with combination of a single neuron recording (whole-cell) and population voltage imaging (genetically encoded voltage indicator) — Jang J, et al., Frontiers in Neuroscience 2021
Cortex-wide dynamics of intrinsic electrical activities: propagating waves and their interactions — Liang Y, Song C, Liu M, Gong P, Zhou C, Knöpfel T, Journal of Neuroscience 2021
Combining cortical voltage imaging and hippocampal electrophysiology for investigating global, multi-timescale activity interactions in the brain — Pedrosa R, et al., International Journal of Molecular Sciences 2022
Evoked cortical depolarizations before and after the amyloid plaque accumulation: voltage imaging study — Zhu MH, Jogdand AH, Jang J, Nagella SC, Das B, Milosevic MM, Yan R, Antic SD, Journal of Alzheimer's Disease 2022
Cortical correlates of psychedelic-induced shaking behavior revealed by voltage imaging — Buchborn T, et al., International Journal of Molecular Sciences 2023
Complexity of cortical wave patterns of the wake mouse cortex — Liang Y, Liang J, Song C, Liu M, Knöpfel T, Gong P, Zhou C, Nature Communications 2023
Imaging of evoked cortical depolarizations using either ASAP2s, or chi-VSFP, or Di-4-Anepps, or autofluorescence optical signals — Milicevic KD, et al., Journal of Integrative Neuroscience 2023
Cortical networks relating to arousal are differentially coupled to neural activity and hemodynamics — Meyer-Baese L, et al., Journal of Neuroscience 2024
Ex vivo propagation of synaptically-evoked cortical depolarizations in a mouse model of Alzheimer's disease at 20 Hz, 40 Hz, or 83 Hz — Patel AA, Zhu MH, Yan R, Antic SD, Scientific Reports 2024
Psychedelic 5-HT2A agonist increases spontaneous and evoked 5-Hz oscillations in visual and retrosplenial cortex — White CM, Azimi Z, Staadt R, Song C, Knöpfel T, Jancke D, Communications Biology 2026