Archon1
Robotically evolved archaerhodopsin variant with improved brightness, millisecond kinetics, and exceptional photostability for long-duration recordings.
Category: Opsin GEVI · Published: 2018 · Nature Chemical Biology 2018
Speed: τ_on=0.61 ms, τ_off=1.1 ms
Dynamic Range: 43% ΔF/F per 100mV
Sensitivity: 30% ΔF/F per AP
Brightness: 80× vs arch
Research Papers (16)
- A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters — Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, Sabatini BL, Boyden ES, Nature Chemical Biology 2018
- Rhodopsin-based voltage imaging tools for use in muscles and neurons of Caenorhabditis elegans — Azimi Hashemi N, Bergs ACF, Schüler C, Scheiwe AR, Steuer Costa W, Bach M, Liewald JF, Gottschalk A, Proceedings of the National Academy of Sciences 2019
- In Vitro Testing of Voltage Indicators: Archon1, ArcLightD, ASAP1, ASAP2s, ASAP3b, Bongwoori-Pos6, BeRST1, FlicR1, and Chi-VSFP-Butterfly — Milosevic MM, Jang J, McKimm EJ, Zhu MH, Antic SD, eNeuro 2020
- Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1 — Shroff SN, Das SL, Tseng HA, Noueihed J, Fernandez F, White JA, Chen CS, Han X, iScience 2020
- High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo — Xie ME, Adam Y, Fan LZ, Böhm UL, Kinsella I, Zhou D, Rozsa M, Singh A, Svoboda K, Paninski L, Cohen AE, Cell Reports 2021
- Plakophilin-2 truncating variants impair cardiac contractility by disrupting sarcomere stability and organization — Zhang K, Cloonan PE, Sundaram S, Liu F, Das SL, Ewoldt JK, Bays JL, Tomp S, Toepfer CN, Marsiglia JDC, Gorham J, Reichart D, Eyckmans J, Seidman JG, Seidman CE, Chen CS, Science Advances 2021
- Studying Synaptically Evoked Cortical Responses ex vivo With Combination of a Single Neuron Recording (Whole-Cell) and Population Voltage Imaging (Genetically Encoded Voltage Indicator) — Jang J, Zhu MH, Jogdand AH, Antic SD, Frontiers in Neuroscience 2021
- Neuronal activity drives pathway-specific depolarization of peripheral astrocyte processes — Armbruster M, Naskar S, Garcia JP, Sommer M, Kim E, Adam Y, Haydon PG, Boyden ES, Cohen AE, Dulla CG, Nature Neuroscience 2022
- Voltage imaging identifies spinal circuits that modulate locomotor adaptation in zebrafish — Böhm UL, Kimura Y, Kawashima T, Ahrens MB, Higashijima S, Engert F, Cohen AE, Neuron 2022
- Video-based pooled screening yields improved far-red genetically encoded voltage indicators — Tian H, Davis HC, Wong-Campos JD, Park P, Fan LZ, Gmeiner B, Begum S, Werley CA, Borja GB, Upadhyay H, Shah H, Jacques J, Qi Y, Parot V, Deisseroth K, Cohen AE, Nature Methods 2023
- Characterization of two near-infrared genetically encoded voltage indicators — Song C, Matlashov ME, Shcherbakova DM, Antic SD, Verkhusha VV, Knöpfel T, Neurophotonics 2024
- Subthreshold electric fields bidirectionally modulate neurotransmitter release through axon polarization — Aberra AS, Miles MW, Hoppa MB, Brain Stimulation 2025
- Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice — Villette V, Chavarha M, Dimov IK, Bradley J, Pradhan L, Mathieu B, Evans SW, Chamberland S, Shi D, Yang R, Kim BB, Ayon A, Jalil A, St-Pierre F, Schnitzer MJ, Bi G, Toth K, Ding J, Dieudonne S, Lin MZ, Cell 2019
- Screening and cellular characterization of genetically encoded voltage indicators based on near-infrared fluorescent proteins — Monakhov MV, Matlashov ME, Colavita M, Song C, Shcherbakova DM, Antic SD, Verkhusha VV, Knopfel T, ACS Chemical Neuroscience 2020
- QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins — Silapetere A, Hwang S, Hontani Y, Fernandez Lahore RG, Balke J, Escobar FV, Tros M, Konold PE, Matis R, Croce R, Walla PJ, Hildebrandt P, Alexiev U, Kennis JTM, Sun H, Utesch T, Hegemann P, Nature Communications 2022
- Detection of Membrane Potential-Dependent Rhodopsin Fluorescence Using Low-Intensity Light Emitting Diode for Long-Term Imaging — Kawanishi S, Kojima K, Shibukawa A, Sakamoto M, Sudo Y, ACS Omega 2023