QuasAr2

Higher-sensitivity QuasAr variant spike counting and subthreshold event detection.

Category: Opsin GEVI · Published: 2014 · Nature Methods 2014

Speed: τ_on=1.2 ms, τ_off=1 ms

Dynamic Range: 90% ΔF/F per 100mV

Sensitivity: 48% ΔF/F per AP

Brightness: 3.4× vs arch

Research Papers (23)

All-optical electrophysiology in mammalian neurons using engineered microbial rhodopsins — Hochbaum DR, Zhao Y, Farhi SL, Klapoetke NC, Werley CA, Kapoor V, Zou P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, Boulting GL, Straub C, Cho YK, Melkonian M, Wong GKS, Harrison DJ, Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE, Nature Methods 2014
Two-photon lifetime imaging of voltage indicating proteins as a probe of absolute membrane voltage — Brinks D, Klein AJ, Cohen AE, Biophysical Journal 2015
Genetically targeted all-optical electrophysiology with a transgenic Cre-dependent Optopatch mouse — Lou S, Adam Y, Weinstein EN, Williams LA, Williams K, Parot V, Kavokine N, Liberles S, Madisen L, Zeng H, Cohen AE, Journal of Neuroscience 2016
Optical electrophysiology for probing function and pharmacology of voltage-gated ion channels — Zhang H, Reichert E, Cohen AE, eLife 2016
Cardiotoxicity screening with simultaneous optogenetic pacing, voltage imaging and calcium imaging — Dempsey GT, Chaudhary KW, Atwater N, Nguyen C, Brown BS, McNeish JD, Cohen AE, Kralj JM, Journal of Pharmacological and Toxicological Methods 2016
All-optical electrophysiology for disease modeling and pharmacological characterization of neurons — Werley CA, Brookings T, Upadhyay H, Williams LA, McManus OB, Dempsey GT, Current Protocols in Pharmacology 2017
Ultrawidefield microscope for high-speed fluorescence imaging and targeted optogenetic stimulation — Werley CA, Chien MP, Cohen AE, Biomedical Optics Express 2017
Geometry-dependent functional changes in iPSC-derived cardiomyocytes probed by functional imaging and RNA sequencing — Werley CA, Chien MP, Gaublomme J, Shekhar K, Butty V, Yi BA, Kralj JM, Bhatt R, Boyer LA, Regev A, Cohen AE, PLOS ONE 2017
Evaluation of optogenetic electrophysiology tools in human stem cell-derived cardiomyocytes — Bjork S, Ojala EA, Nordstrom T, Ahola A, Liljestrom M, Hyttinen J, Kankuri E, Mervaala E, Frontiers in Physiology 2017
A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice — Yi B, Bhatt D, Bhatt R, Baker BJ, Scientific Reports 2018
All-optical electrophysiology for high-throughput functional characterization of a human iPSC-derived motor neuron model of ALS — Kiskinis E, Kralj JM, Zou P, Weinstein EN, Zhang H, Tsioras K, Wiskow O, Ortega JA, Eggan K, Cohen AE, Stem Cell Reports 2018
Optical interrogation of neuronal circuitry in zebrafish using genetically encoded voltage indicators — Miyazawa H, Okumura K, Hiyoshi K, Maruyama K, Kakinuma H, Amo R, Okamoto H, Yamasu K, Tsuda S, Scientific Reports 2018
Comparative evaluation of genetically encoded voltage indicators — Bando Y, Sakamoto M, Kim S, Ayzenshtat I, Yuste R, Cell Reports 2019
Electrophysiological characterization of drug response in hSC-derived cardiomyocytes using voltage-sensitive optical platforms — Pfeiffer-Kaushik ER, Smith GL, Cai B, Dempsey GT, Bhatt D, Bhatt R, Hortigon-Vinagre MP, Zamora V, Smith G, Journal of Pharmacological and Toxicological Methods 2019
Simultaneous voltage and calcium imaging and optogenetic stimulation with high sensitivity and a wide field of view — Nguyen C, Bhatt D, Bhatt R, Rozsahegyi EJ, Bhatt R, Werley CA, Biomedical Optics Express 2019
Rhodopsin-based voltage imaging tools for use in muscles and neurons of Caenorhabditis elegans — Azimi Hashemi N, Bergs ACF, Schuler C, Scheiwe AR, Steuer Costa W, Bach M, Liewald JF, Gottschalk A, Proceedings of the National Academy of Sciences 2019
Highly parallelized, multicolor optogenetic recordings of cellular activity for therapeutic discovery applications in ion channels and disease-associated excitable cells — Borja G, Zhang H, Harwood J, Jacques K, Bhatt D, Bhatt R, Bhatt R, Werley CA, Frontiers in Molecular Neuroscience 2022
Human cancer cells generate spontaneous calcium transients and intercellular waves that modulate tumor growth — Liang H, Zhang C, Chen H, Liu T, Tanaka T, Wang Y, Biomaterials 2022
All-optical closed-loop voltage clamp for precise control of muscles and neurons in live animals — Bergs ACF, Liewald JF, Rodriguez-Rozada S, Liu Q, Wirt C, Bessel A, Zeitzschel N, Durmaz H, Nozownik A, Dill H, Jospin M, Vierock J, Bargmann CI, Hegemann P, Wiegert JS, Gottschalk A, Nature Communications 2023
A phenotypic screening platform for chronic pain therapeutics using all-optical electrophysiology — Liu S, Zhang H, Werley CA, Bhatt D, Bhatt R, Bhatt R, Bhatt D, Bhatt R, PAIN 2024
A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters — Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Flavell SW, Sabatini BL, Boyden ES, Nature Chemical Biology 2018
Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice — Villette V, Chavarha M, Dimov IK, Bradley J, Pradhan L, Mathieu B, Evans SW, Chamberland S, Shi D, Yang R, Kim BB, Ayon A, Jalil A, St-Pierre F, Schnitzer MJ, Bi G, Toth K, Ding J, Dieudonne S, Lin MZ, Cell 2019
Screening and cellular characterization of genetically encoded voltage indicators based on near-infrared fluorescent proteins — Monakhov MV, Matlashov ME, Colavita M, Song C, Shcherbakova DM, Antic SD, Verkhusha VV, Knopfel T, ACS Chemical Neuroscience 2020