Archon2

Sibling variant of Archon1 with superior membrane localization and brightness at the cost of reduced sensitivity.

Category: Opsin GEVI · Published: 2018 · Nature Chemical Biology 2018

Speed: τ_on=0.06 ms, τ_off=0.17 ms

Dynamic Range: 20% ΔF/F per 100mV

Sensitivity: 18% ΔF/F per AP

Brightness: 28× vs arch

Research Papers (4)

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters — Piatkevich KD, Jung EE, Straub C, Linghu C, Park D, Suk HJ, Hochbaum DR, Goodwin D, Pnevmatikakis E, Pak N, Kawashima T, Yang CT, Rhoades JL, Shemesh O, Asano S, Yoon YG, Freifeld L, Saulnier JL, Riegler C, Engert F, Hughes T, Drobizhev M, Szabo B, Ahrens MB, Bhatt DH, Schreiter ER, Sabatini BL, Bhatt DH, Boyden ES, Nature Chemical Biology 2018
Screening and Cellular Characterization of Genetically Encoded Voltage Indicators Based on Near-Infrared Fluorescent Proteins — Monakhov MV, Matlashov ME, Colavita M, Song C, Shcherbakova DM, Antic SD, Bhatt DH, Verkhusha VV, Knöpfel T, ACS Chemical Neuroscience 2020
Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor Archon2 — Penzkofer A, Silapetere A, Hegemann P, International Journal of Molecular Sciences 2020
Photocycle dynamics of the Archaerhodopsin 3 based fluorescent voltage sensor Archon2 — Penzkofer A, Silapetere A, Hegemann P, Journal of Photochemistry and Photobiology B: Biology 2021