ASAP3
3rd-gen ASAP sensor with large fluorescence modulation and fast kinetics.
Category: VSD-cpGFP GEVI · Published: 2019 · Cell 2019
Speed: τ_on=0.94 ms, τ_off=3.79 ms
Dynamic Range: -51% ΔF/F per 100mV
Sensitivity: 18% ΔF/F per AP
Brightness: 0.748× vs EGFP
Research Papers (22)
- Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice — Villette V, Chavarha M, et al., Cell 2019
- Two-Photon Voltage Imaging of Spontaneous Activity from Multiple Neurons Reveals Network Activity in Brain Tissue — Li B, et al., iScience 2020
- In Vitro Testing of Voltage Indicators: Archon1, ArcLightD, ASAP1, ASAP2s, ASAP3b, Bongwoori-Pos6, BeRST1, FlicR1, and Chi-VSFP-Butterfly — Milosevic MM, et al., eNeuro 2020
- A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3 — Kim BB, et al., Scientific Reports 2022
- Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy — Liu Z, et al., Cell 2022
- Electrical signals in the ER are cell type and stimulus specific with extreme spatial compartmentalization in neurons — Campbell EP, et al., Cell Reports 2023
- High-speed low-light in vivo two-photon voltage imaging of large neuronal populations — Platisa J, et al., Nature Methods 2023
- A positively tuned voltage indicator for extended electrical recordings in the brain — Evans SW, et al., Nature Methods 2023
- Dendritic mGluR2 and perisomatic Kv3 signaling regulate dendritic computation of mouse starburst amacrine cells — Ledesma HA, et al., Nature Communications 2024
- Two-dimensional electro-optical multiphoton microscopy — Farinella DM, et al., Neurophotonics 2024
- Cl⁻-dependent amplification of excitatory synaptic potentials at distal dendrites revealed by voltage imaging — Higashi R, et al., Science Advances 2024
- A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events — Hao YA, et al., Neuron 2024
- Volumetric voltage imaging of neuronal populations in the mouse brain by confocal light-field microscopy — Bai L, et al., Nature Methods 2024
- Development and Application of a Mitochondrial Genetically Encoded Voltage Indicator in Narcosis — Yang RZ, et al., Neuroscience Bulletin 2024
- An Ultrasensitive Genetically Encoded Voltage Indicator Uncovers the Electrical Activity of Non-Excitable Cells — Rühl P, et al., Advanced Science 2024
- Functional architecture of intracellular oscillations in hippocampal dendrites — Liao Z, et al., Nature Communications 2024
- Absolute Membrane Potential Recording with ASAP-Type Genetically Encoded Voltage Indicators Using Fluorescence Lifetime Imaging — Nair AG, et al., ACS Chemical Neuroscience 2025
- Voltage imaging reveals hippocampal inhibitory dynamics shaping pyramidal memory-encoding sequences — Taxidis J, et al., Nature Neuroscience 2025
- An axonal brake on striatal dopamine output by cholinergic interneurons — Zhang YF, et al., Nature Neuroscience 2025
- Imaging high-frequency voltage dynamics in multiple neuron classes of behaving mammals — Haziza S, et al., Cell 2025
- Neuronal activity induces myelin voltage changes that reflect action potential dependent myelin potassium buffering — Labarchède M, et al., bioRxiv 2025
- Movement-stabilized three-dimensional optical recordings of membrane potential changes and calcium dynamics in hippocampal CA1 dendrites — Gonzalez KC, et al., Neuron 2026