ASAP4e
Speed-optimized ASAP4 variant with fast kinetics and a large fluorescence increase per action potential.
Category: VSD-cpGFP GEVI · Published: 2023 · Nature Methods 2023
Speed: τ_on=18.29 ms, τ_off=15.6 ms
Dynamic Range: 210% ΔF/F per 100mV
Sensitivity: 38% ΔF/F per AP
Brightness: 0.362× vs asap3
Research Papers (6)
- A positively tuned voltage indicator for extended electrical recordings in the brain — Evans SW, Shi DQ, Chavarha M, et al., Nature Methods 2023
- Improving positively tuned voltage indicators for faster kinetics and higher contrast — Lee S, Zhang G, Hao YA, et al., bioRxiv 2024
- A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo — McDonald AJ, Land MA, Yang S, et al., bioRxiv 2026
- Voltage imaging reveals hippocampal inhibitory dynamics shaping pyramidal memory-encoding sequences — Taxidis J, et al., Nature Neuroscience 2025
- Imaging sensory transmission and neuronal plasticity in primary sensory neurons with a positively tuned voltage indicator — Zhang Y, et al., Nature Communications 2025
- Retroreflective beam multiplexing enables high-throughput two-photon voltage imaging in vivo — Liu Z, Han M, Chen Y, Downs NP, Wu J, bioRxiv 2025