HArclight1
Chemigenetic adaptation of the ArcLight architecture that replaces the pH-sensitive GFP with a HaloTag labeled with a far-red Si-rhodamine dye, giving negative-going fluorescence responses to depolarization.
Category: Chemigenetic GEVI · Published: 2021 · Nature Chemical Biology 2021
Speed: τ_on=2.2 ms, τ_off=1.6 ms
Dynamic Range: -3.5% ΔF/F per 100mV
Brightness: 3.03× vs EGFP
Research Papers (1)
- The HaloTag as a general scaffold for far-red tunable chemigenetic indicators — Deo C, Abdelfattah AS, Bhargava HK, Berro AJ, Falco N, Farrants H, Moeyaert B, Chupanova M, Lavis LD, Schreiter ER, Nature Chemical Biology 2021