HASAP1

Chemigenetic adaptation of the ASAP architecture that replaces cpGFP with cpHaloTag labeled with a far-red Si-rhodamine dye, giving positive-going fluorescence responses to depolarization.

Category: Chemigenetic GEVI · Published: 2021 · Nature Chemical Biology 2021

Speed: τ_on=2.1 ms, τ_off=1.1 ms

Dynamic Range: 9.3% ΔF/F per 100mV

Brightness: 3.03× vs EGFP

Research Papers (1)

The HaloTag as a general scaffold for far-red tunable chemigenetic indicators — Deo C, Abdelfattah AS, Bhargava HK, Berro AJ, Falco N, Farrants H, Moeyaert B, Chupanova M, Lavis LD, Schreiter ER, Nature Chemical Biology 2021