JEDI-2P
Two-photon optimized GEVI evolved from ASAP2s with improved brightness, photostability, and sensitivity under two-photon illumination.
Category: VSD-cpGFP GEVI · Published: 2022 · Cell 2022
Speed: τ_on=0.54 ms, τ_off=1.21 ms
Dynamic Range: -43% ΔF/F per 100mV
Sensitivity: 18.2% ΔF/F per AP
Brightness: 1.67× vs asap3
Research Papers (13)
- Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy — Liu Z, Lu X, Villette V, et al., Cell 2022
- Scanless two-photon voltage imaging — Sims RR, et al., Nature Communications 2024
- A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events — Hao YA, et al., Neuron 2024
- A novel rhodopsin-based voltage indicator for simultaneous two-photon optical recording with GCaMP in vivo — Villette V, et al., bioRxiv 2024
- Two-photon voltage imaging with rhodopsin-based sensors — Grimm C, et al., Neuron 2026
- All-optical voltage interrogation for probing synaptic plasticity in vivo — Carolan J, et al., Nature Communications 2025
- FACED 2.0 enables large-scale voltage and calcium imaging in vivo — Zhong J, et al., Nature Methods 2025
- Retroreflective beam multiplexing enables high-throughput two-photon voltage imaging in vivo — Liu Z, et al., bioRxiv 2025
- Movement-stabilized three-dimensional optical recordings of membrane potential changes and calcium dynamics in hippocampal CA1 dendrites — Gonzalez KC, et al., Neuron 2026
- Kilohertz-rate two-photon voltage imaging of population dynamics in vivo — Zhang M, et al., bioRxiv 2026
- Designer indicators for two-photon recording of subthreshold voltage dynamics — Land MA, Galdamez M, Villette V, Zhu J, Lu X, Marosi M, et al., Nature Methods 2026
- Optical constraints on two-photon voltage imaging — Brooks FP, et al., Neurophotonics 2024
- Linking functional activity and connectivity of neuronal circuits via fast cross-layer all-optical physiology — Liu C, et al., Cell Reports 2025