GEVIBench › All sensors › JEDI-3hyp
JEDI-3hyp
JEDI-2P variant with two-photon voltage sensitivity shifted toward hyperpolarized potentials for detecting subthreshold fluctuations below resting membrane potential.
- Category: VSD-cpGFP GEVI
- Published: 2026-04-15 — Nature Methods 2026
- Derived from: JEDI-2P
- Response polarity: negative-going (fluorescence decreases on depolarization)
- Spectrum: excitation peak 501 nm, emission peak 519 nm (JEDI-3hyp)
- Two-photon compatible: yes
- Plasmid: Addgene #246631
- Tags: cpGFP, Two-photon
Headline values (as shown in the GEVIBench table)
| Metric | Value | Definition |
|---|---|---|
| Brightness (B/B_EGFP) | 0.41× EGFP | Molecular brightness relative to EGFP (extinction coefficient × quantum yield, or a measured ratio). Sensors not measured against EGFP directly are placed through a chain of pairwise brightness comparisons, combined by geometric mean. |
| Kinetics τ_on / τ_off (ms) | 1.2 / 2.1 ms | Activation / decay time constants in ms (amplitude-weighted mean τ for multi-exponential fits). The value shown averages the measurements made at physiological temperature (≥33 °C); if there are none, it is the measurement closest to 33 °C. |
| ΔF/F per 100 mV | -51.5% (median of 2 measurements: −51.6%) | Steady-state fluorescence change for a 100 mV depolarization step (−70 → +30 mV); the sign gives the polarity. The table shows the first listed measurement; the median across measurements (one-photon preferred) is used for sorting. |
| ΔF/F per mV | 1.9% (estimated from the F–V curve) | Subthreshold sensitivity: fluorescence change per mV near rest (−90 to −50 mV). Where no value is reported directly, it is estimated from the F–V curve. |
| ΔF/F per AP | 36.4% | Fluorescence change per single action potential in neurons. The table shows the first listed measurement; the median across measurements (one-photon preferred) is used for sorting. |
| AP width (FWHM, ms) | 3.8 ms | Full width at half maximum of the optical spike waveform. |
| Photostability t75% @ 100 mW/mm² | — | Time for fluorescence to fall to 75% of its initial value under one-photon illumination, linearly dose-scaled to 100 mW/mm² (t50% is used where the 75% crossing falls inside a fast initial transient). Two-photon curves are not dose-scaled and are excluded. |
| Papers using it (N_used) | 1 | Number of published research papers, tracked by GEVIBench, that used the sensor for an experiment (a popularity / track-record measure). |
Measurements and sources
Kinetics
- τ_on 1.21 ms, τ_off 2.11 ms at 33°C — Land 2026, Table 1
ΔF/F per 100 mV
- -51.5%, 2P — Land 2026, Fig. 1h
- -51.72%, 2P — Land 2026, Ext. Fig. 1b
ΔF/F per action potential
- 36.4%, 2P — Land 2026, Fig. 1j
AP width (FWHM)
- 6.3 ms at 20000 Hz sampling, HEK293A cells, whole-cell voltage clamp, in vitro (2-ms FWHM command AP waveform), 2P, 23°C — Land 2026, Fig. 1k
- 1.2 ms at 7000 Hz sampling, Mouse visual cortex, awake behaving, in vivo, soma-targeted JEDI3hyp-Kv (ULoVE 2P), 2P, 37°C — Land 2026, Fig. 2d
Brightness comparisons
- 0.86× the brightness of JEDI-2P — Land 2026, Fig. 1d
- 1.25× the brightness of ASAP5 — Land 2026, Ext. Fig. 1c
Photobleaching
- t75% = 83 s at 37 mW at sample plane (2P), 2P — Land 2026, Fig. 1e
Fluorescence–voltage (F–V) curve
ΔF/F (%) at each membrane potential, normalized to 0 at −70 mV: -120 mV: 91%; -100 mV: 65%; -80 mV: 20%; -70 mV: 0%; -60 mV: -18%; -50 mV: -28%; -30 mV: -40%; 0 mV: -45%; 30 mV: -52%; 50 mV: -55% — Land 2026, Fig. 1h
Research papers using JEDI-3hyp (1)
- Designer indicators for two-photon recording of subthreshold voltage dynamics — Land MA, Galdamez M, Villette V, Zhu J, Lu X, Marosi M, et al., Nature Methods 2026 (HEK293 cells, in vitro; Rat, cultured hippocampal neurons, in vitro; Mouse, visual cortex L2/3, in vivo; Mouse, hippocampus CA1 PV+ interneurons, in vivo; Mouse, cortex VIP+/SOM+ interneurons, in vivo)
Data and citation
- llms.txt — summary for language models
- llms-full.txt — every sensor in full (Markdown)
- gevis.json — all values and measurements with sources (JSON)
- gevis.csv — one row per sensor (CSV)
Cite GEVIBench as: Adam E. Cohen and Daozheng Gong (2026). Voltage imaging: A practical guide. Neuron. https://doi.org/10.1016/j.neuron.2026.08.002
Generated from the GEVIBench database on 2026-10-12.